| Display Channel | Digital Channel | Call Sign | Network | Nickname | Hours | Notes |
| KMOS-TV | SEDALIA, MO | |||||
| 06-1 | 15.3 | KMOS-TV | PBS | "KMOS" | ||
| 06-2 | 15.4 | Create | Create | |||
| 06-3 | 15.5 | Emerge | PBS Encore | "KMOS Emerge" | ||
| 06-4 | 15.6 | KIDS | PBS Kids 24/7 | |||
| KOMU-TV | COLUMBIA, MO | |||||
| 08-1 | 27.3 | KOMU-DT | NBC | "KOMU 8" | ||
| 08-3 | 27.5 | KOMU-CW | CW+ | "Mid-Missouri CW" | ||
| KRCG | JEFFERSON CITY, MO | |||||
| 13-1 | 29.3 | CBS | CBS | "KRCG 13" | ||
| 13-2 | 29.4 | Comet | Comet TV | |||
| 13-3 | 29.5 | Charge! | Charge! | |||
| 13-4 | 29.6 | TBD | TBD | |||
| KMIZ | COLUMBIA, MO | |||||
| 17-1 | 17.3 | KMIZ-DT | ABC | "ABC 17" | ||
| 17-2 | 17.4 | Me-TV | Me-TV | |||
| 17-3 | 17.5 | KZOU-TV | MyN | "My ZOU" | ||
| 17-4 | 17.6 | KQFX | FOX | "Fox 22" | ||
| 17-5 | 17.7 | Bounce | Bounce TV | |||
| 17-6 | 17.8 | Nosey | Nosey | |||
| 17-7 | 17.9 | Confess | Confess | |||
| K18KK-D | COLUMBIA, MO | |||||
| 22-1 | 18.3 | FOX 22 | FOX | "Fox 22" | ||
| KQFX-LD | COLUMBIA, MO | |||||
| 22-1 | 30.3 | FOX 22 | FOX | "Fox 22" | ||
| K18KK-D | COLUMBIA, MO | |||||
| 22-2 | 18.4 | Laff | Laff | |||
| KQFX-LD | COLUMBIA, MO | |||||
| 22-2 | 30.4 | Laff | Laff | |||
| 22-3 | 30.5 | Grit | Grit | |||
| K18KK-D | COLUMBIA, MO | |||||
| 22-3 | 18.5 | Grit | Grit | |||
| KQFX-LD | COLUMBIA, MO | |||||
| 22-4 | 30.6 | Mystery | ION Mystery | |||
| K18KK-D | COLUMBIA, MO | |||||
| 22-4 | 18.6 | Mystery | ION Mystery | |||
| 22-5 | 18.7 | DABL | Dabl | |||
| KQFX-LD | COLUMBIA, MO | |||||
| 22-5 | 30.7 | DABL | Dabl | |||
| KFDR | JEFFERSON CITY, MO | |||||
| 25-1 | 20.3 | KFDR-HD | CTN | |||
| 25-2 | 20.4 | Lifesty | CTN Lifestyle | |||
| 25-3 | 20.5 | CTNi | CTNi | |||
| 25-5 | 20.7 | BIZ-TV | Biz TV | |||
| KRMS-LD | LAKE OZARK, MO | |||||
| 32-01 | 32.6 | COZITV | COZI TV | |||
| 32-02 | 32.4 | RAVNEWS | America's Voice | |||
| 32-03 | 32.2 | OAN | One America Plus | |||
| 32-04 | 32.8 | GETTV | GET | |||
| 32-05 | 32.10 | BUZZR | BUZZR | |||
| 32-06 | 32.12 | YTA | YouToo America | |||
| 32-07 | 32.14 | NOST | Nostalgia Network | |||
| 32-08 | 32.16 | DAYSTAR | Daystar | |||
| 32-09 | 32.18 | WEATHER | WeatherNation | |||
| 32-10 | 32.20 | TCN | The Country Network | |||
| 32-11 | 32.22 | KRMS | Local | |||
| K33MN-D | JEFFERSON CITY, MO | |||||
| 33-1 | 33.1 | K33MN-D | ||||
| K35OY-D | COLUMBIA, MO | |||||
| 35-1 | 35.1001 | K35OY-D | The365 | |||
| 35-2 | 35.1002 | K35OY-D | American Crimes | |||
| 35-3 | 35.1003 | K35OY-D | Oxygen | |||
| 35-4 | 35.1004 | K35OY-D | Outlaw | |||
| 35-5 | 35.1005 | K35OY-D | Defy TV | |||
| 35-6 | 35.1006 | K35OY-D | Salem News Channel | |||
| 35-7 | 35.1007 | K35OY-D | ||||
| KGKM-LD | COLUMBIA, MO | |||||
| 36-1 | 36.1 | KGKM-LD | Telemundo | |||
| 36-2 | 36.2 | ION | ION | |||
| 36-3 | 36.3 | COURTTV | Court TV | |||
| 36-4 | 36.4 | IONPlus | ION Plus | |||
| 36-5 | 36.5 | GRIT | Grit | |||
| 36-6 | 36.6 | Mystery | ION Mystery | |||
| 36-7 | 36.7 | SONLIFE | SonLife | |||