| City | Population |
| Ackworth, IA | 83 |
| Adair, IA | 781 |
| Adair County rural, IA | 2,009 |
| Adel, IA | 3,682 |
| Afton, IA | 845 |
| Albia, IA | 3,766 |
| Albion, IA | 505 |
| Alden, IA | 787 |
| Alexander, IA | 175 |
| Alleman, IA | 432 |
| Allerton, IA | 501 |
| Altoona, IA | 14,541 |
| Ames, IA | 58,965 |
| Ankeny, IA | 45,582 |
| Arcadia, IA | 484 |
| Arispe, IA | 100 |
| Auburn, IA | 322 |
| Audubon, IA | 2,176 |
| Audubon County rural, IA | 2,590 |
| Badger, IA | 561 |
| Bagley, IA | 303 |
| Barnes City, IA | 176 |
| Barnum, IA | 191 |
| Baxter, IA | 1,101 |
| Bayard, IA | 471 |
| Beacon, IA | 494 |
| Beaconsfield, IA | 15 |
| Beaman, IA | 191 |
| Beaver, IA | 48 |
| Belmond, IA | 2,376 |
| Benton, IA | 41 |
| Berkley, IA | 32 |
| Bevington, IA | 63 |
| Blairsburg, IA | 215 |
| Blakesburg, IA | 296 |
| Bode, IA | 302 |
| Bondurant, IA | 3,860 |
| Boone, IA | 12,661 |
| Boone County rural, IA | 8,386 |
| Bouton, IA | 129 |
| Boxholm, IA | 195 |
| Bradford, IA | 99 |
| Bradgate, IA | 86 |
| Breda, IA | 483 |
| Bridgewater, IA | 182 |
| Buckeye, IA | 108 |
| Bussey, IA | 422 |
| Calhoun County rural, IA | 2,509 |
| Callender, IA | 376 |
| Cambridge, IA | 827 |
| Carlisle, IA | 3,876 |
| Carroll, IA | 10,103 |
| Carroll County rural, IA | 4,453 |
| Casey, IA | 426 |
| Chariton, IA | 4,321 |
| Chillicothe, IA | 97 |
| Churdan, IA | 386 |
| Clare, IA | 146 |
| Clarion, IA | 2,850 |
| Clarke County rural, IA | 3,399 |
| Clearfield, IA | 363 |
| Clemons, IA | 148 |
| Clio, IA | 80 |
| Clive, IA | 15,447 |
| Coalville, IA | 610 |
| Colfax, IA | 2,093 |
| Collins, IA | 495 |
| Colo, IA | 876 |
| Conrad, IA | 1,108 |
| Coon Rapids, IA | 1,305 |
| Corwith, IA | 309 |
| Corydon, IA | 1,585 |
| Coulter, IA | 281 |
| Creston, IA | 7,834 |
| Cromwell, IA | 107 |
| Cumming, IA | 351 |
| Dakota City, IA | 843 |
| Dallas Center, IA | 1,623 |
| Dallas County rural, IA | 30,799 |
| Dana, IA | 71 |
| Davis City, IA | 204 |
| Dawson, IA | 131 |
| Dayton, IA | 837 |
| De Soto, IA | 1,050 |
| Decatur City, IA | 197 |
| Decatur County rural, IA | 2,889 |
| Dedham, IA | 266 |
| Delphos, IA | 25 |
| Derby, IA | 115 |
| Des Moines, IA | 203,433 |
| Dexter, IA | 611 |
| Diagonal, IA | 330 |
| Diamondhead Lake, IA | 366 |
| Dows, IA | 538 |
| Duncombe, IA | 410 |
| Eagle Grove, IA | 3,583 |
| Earlham, IA | 1,450 |
| East Peru, IA | 125 |
| Eddyville, IA | 1,024 |
| Eldora, IA | 2,732 |
| Elkhart, IA | 683 |
| Ellston, IA | 43 |
| Ellsworth, IA | 531 |
| Farnhamville, IA | 371 |
| Ferguson, IA | 126 |
| Fontanelle, IA | 672 |
| Fort Dodge, IA | 25,206 |
| Fraser, IA | 102 |
| Fremont, IA | 743 |
| Galt, IA | 32 |
| Garden City, IA | 89 |
| Garden Grove, IA | 211 |
| Geneva, IA | 165 |
| Gilbert, IA | 1,082 |
| Gilman, IA | 509 |
| Gilmore City, IA | 504 |
| Glidden, IA | 1,146 |
| Goldfield, IA | 635 |
| Gowrie, IA | 1,037 |
| Grand Junction, IA | 824 |
| Grand River, IA | 236 |
| Granger, IA | 1,244 |
| Green Mountain, IA | 126 |
| Greene County rural, IA | 2,625 |
| Greenfield, IA | 1,982 |
| Grimes, IA | 8,246 |
| Grinnell, IA | 9,218 |
| Guthrie Center, IA | 1,569 |
| Guthrie County rural, IA | 4,563 |
| Halbur, IA | 246 |
| Hamilton, IA | 130 |
| Hamilton County rural, IA | 3,761 |
| Harcourt, IA | 303 |
| Hardin County rural, IA | 4,474 |
| Hardy, IA | 47 |
| Hartford, IA | 771 |
| Harvey, IA | 235 |
| Haverhill, IA | 173 |
| Hubbard, IA | 845 |
| Humboldt, IA | 4,690 |
| Humboldt County rural, IA | 2,327 |
| Humeston, IA | 494 |
| Huxley, IA | 3,317 |
| Indianola, IA | 14,782 |
| Iowa Falls, IA | 5,238 |
| Irvington, IA | 38 |
| Jamaica, IA | 224 |
| Jasper County rural, IA | 12,246 |
| Jefferson, IA | 4,345 |
| Jewell Junction, IA | 1,215 |
| Johnston, IA | 17,278 |
| Jolley, IA | 41 |
| Kamrar, IA | 199 |
| Kanawha, IA | 652 |
| Kellerton, IA | 315 |
| Kelley, IA | 309 |
| Kellogg, IA | 599 |
| Kent, IA | 61 |
| Keomah Village, IA | 84 |
| Kirkville, IA | 167 |
| Knierim, IA | 60 |
| Knoxville, IA | 7,313 |
| Lacona, IA | 361 |
| Lake City, IA | 1,727 |
| Lake Panorama, IA | 1,309 |
| Lake View, IA | 1,142 |
| Lambs Grove, IA | 172 |
| Lamoni, IA | 2,324 |
| Lanesboro, IA | 121 |
| Latimer, IA | 507 |
| Laurel, IA | 239 |
| Le Grand, IA | 938 |
| Le Roy, IA | 15 |
| Lehigh, IA | 416 |
| Leighton, IA | 162 |
| Lenox, IA | 1,407 |
| Leon, IA | 1,977 |
| Lidderdale, IA | 180 |
| Linden, IA | 199 |
| Lineville, IA | 217 |
| Liscomb, IA | 301 |
| Livermore, IA | 384 |
| Lohrville, IA | 368 |
| Lorimor, IA | 360 |
| Lovilia, IA | 538 |
| Lu Verne, IA | 261 |
| Lucas, IA | 216 |
| Lucas County rural, IA | 3,540 |
| Luther, IA | 122 |
| Lynnville, IA | 379 |
| Lytton, IA | 315 |
| Macksburg, IA | 113 |
| Madison County rural, IA | 7,533 |
| Madrid, IA | 2,543 |
| Mahaska County rural, IA | 7,311 |
| Malcom, IA | 287 |
| Maloy, IA | 29 |
| Manson, IA | 1,690 |
| Marion County rural, IA | 11,737 |
| Marshall County rural, IA | 7,326 |
| Marshalltown, IA | 27,552 |
| Martensdale, IA | 465 |
| Marysville, IA | 66 |
| Maxwell, IA | 920 |
| McCallsburg, IA | 333 |
| Melbourne, IA | 830 |
| Melcher-Dallas, IA | 1,288 |
| Melrose, IA | 112 |
| Menlo, IA | 353 |
| Millerton, IA | 45 |
| Milo, IA | 775 |
| Minburn, IA | 365 |
| Mingo, IA | 302 |
| Mitchellville, IA | 2,254 |
| Monroe, IA | 1,830 |
| Monroe County rural, IA | 3,554 |
| Montezuma, IA | 1,462 |
| Moorland, IA | 169 |
| Moravia, IA | 665 |
| Mount Ayr, IA | 1,691 |
| Murray, IA | 756 |
| Mystic, IA | 425 |
| Nevada, IA | 6,798 |
| New Providence, IA | 228 |
| New Sharon, IA | 1,293 |
| New Virginia, IA | 489 |
| Newton, IA | 15,254 |
| Norwalk, IA | 8,945 |
| Oakland Acres, IA | 156 |
| Ogden, IA | 2,044 |
| Orient, IA | 408 |
| Osceola, IA | 4,929 |
| Oskaloosa, IA | 11,463 |
| Otho, IA | 542 |
| Ottosen, IA | 55 |
| Owasa, IA | 43 |
| Palmer, IA | 165 |
| Panora, IA | 1,124 |
| Paton, IA | 236 |
| Patterson, IA | 130 |
| Pella, IA | 10,352 |
| Perry, IA | 7,702 |
| Pilot Mound, IA | 173 |
| Pioneer, IA | 23 |
| Pleasant Hill, IA | 8,785 |
| Pleasanton, IA | 49 |
| Pleasantville, IA | 1,694 |
| Plover, IA | 77 |
| Pocahontas, IA | 1,789 |
| Pocahontas County rural, IA | 2,597 |
| Polk City, IA | 3,418 |
| Pomeroy, IA | 662 |
| Popejoy, IA | 79 |
| Prairie City, IA | 1,680 |
| Promise City, IA | 111 |
| Radcliffe, IA | 545 |
| Ralston, IA | 79 |
| Randall, IA | 173 |
| Rathbun, IA | 89 |
| Reasnor, IA | 152 |
| Redfield, IA | 835 |
| Renwick, IA | 242 |
| Rhodes, IA | 305 |
| Rinard, IA | 52 |
| Ringgold County rural, IA | 2,215 |
| Rippey, IA | 292 |
| Rockwell City, IA | 1,709 |
| Rodman, IA | 45 |
| Roland, IA | 1,284 |
| Rolfe, IA | 584 |
| Rose Hill, IA | 168 |
| Rowan, IA | 158 |
| Runnells, IA | 507 |
| Russell, IA | 554 |
| Rutland, IA | 126 |
| Sandyville, IA | 51 |
| Saylorville, IA | 3,301 |
| Scranton, IA | 557 |
| Searsboro, IA | 148 |
| Sexton, IA | 37 |
| Shannon City, IA | 71 |
| Sheldahl, IA | 319 |
| Slater, IA | 1,489 |
| Somers, IA | 113 |
| Spring Hill, IA | 63 |
| St. Anthony, IA | 102 |
| St. Benedict, IA | 39 |
| St. Charles, IA | 653 |
| St. Joseph, IA | 61 |
| St. Marys, IA | 127 |
| Stanhope, IA | 422 |
| State Center, IA | 1,468 |
| Steamboat Rock, IA | 310 |
| Story City, IA | 3,431 |
| Story County rural, IA | 8,543 |
| Stratford, IA | 743 |
| Stuart, IA | 1,648 |
| Sully, IA | 821 |
| Sun Valley Lake, IA | 161 |
| Swan, IA | 72 |
| Templeton, IA | 362 |
| Thayer, IA | 59 |
| Thor, IA | 186 |
| Tingley, IA | 184 |
| Truro, IA | 485 |
| Twin Lakes, IA | 334 |
| Union, IA | 397 |
| Union County rural, IA | 3,097 |
| University Park, IA | 487 |
| Urbandale, IA | 39,463 |
| Valeria, IA | 57 |
| Van Meter, IA | 1,016 |
| Van Wert, IA | 230 |
| Vincent, IA | 174 |
| Wall Lake, IA | 819 |
| Warren County rural, IA | 18,899 |
| Waukee, IA | 13,790 |
| Wayne County rural, IA | 2,669 |
| Webster City, IA | 8,070 |
| Webster County rural, IA | 7,035 |
| Weldon, IA | 125 |
| Wellsburg, IA | 707 |
| West Bend, IA | 785 |
| West Des Moines, IA | 56,609 |
| Westside, IA | 299 |
| Whittemore, IA | 504 |
| Whitten, IA | 149 |
| Willey, IA | 88 |
| Williams, IA | 344 |
| Williamson, IA | 152 |
| Windsor Heights, IA | 4,860 |
| Winterset, IA | 5,190 |
| Woodburn, IA | 202 |
| Woodward, IA | 1,024 |
| Woolstock, IA | 168 |
| Wright County rural, IA | 2,889 |
| Yale, IA | 246 |
| Yetter, IA | 34 |
| Zearing, IA | 554 |
| | |
| Total | 1,049,802 |
