| City | Population |
| Alva, FL | 2,596 |
| Arcadia, FL | 7,637 |
| Bokeelia, FL | 1,780 |
| Bonita Springs, FL | 43,914 |
| Buckingham, FL | 4,036 |
| Burnt Store Marina, FL | 1,793 |
| Cape Coral, FL | 154,305 |
| Captiva, FL | 583 |
| Charleston Park, FL | 218 |
| Charlotte County rural, FL | 64,395 |
| Charlotte Harbor, FL | 3,714 |
| Charlotte Park, FL | 2,325 |
| Chokoloskee, FL | 359 |
| Cleveland, FL | 2,990 |
| Clewiston, FL | 7,155 |
| Collier County rural, FL | 195,778 |
| Cypress Lake, FL | 11,846 |
| DeSoto County rural, FL | 20,671 |
| Englewood, FL | 14,863 |
| Estero, FL | 22,612 |
| Everglades, FL | 400 |
| Fort Denaud, FL | 1,694 |
| Fort Myers, FL | 62,298 |
| Fort Myers Beach town, FL | 6,277 |
| Fort Myers Shores, FL | 5,487 |
| Gardner, FL | 463 |
| Gateway, FL | 8,401 |
| Glades County rural, FL | 9,754 |
| Golden Gate, FL | 23,961 |
| Goodland, FL | 267 |
| Grove City, FL | 1,804 |
| Harbour Heights, FL | 2,987 |
| Harlem, FL | 2,658 |
| Harlem Heights, FL | 1,975 |
| Hendry County rural, FL | 15,483 |
| Highlands County rural, FL | 77,236 |
| Immokalee, FL | 24,154 |
| Iona, FL | 15,369 |
| Island Walk, FL | 3,035 |
| LaBelle, FL | 4,640 |
| Lake Placid town, FL | 2,223 |
| Laurel, FL | 8,171 |
| Lee County rural, FL | 69,759 |
| Lehigh Acres, FL | 86,784 |
| Lely, FL | 3,451 |
| Lely Resort, FL | 4,646 |
| Limestone, FL | 132 |
| Lochmoor Waterway Estates, FL | 4,204 |
| Manasota Key, FL | 1,229 |
| Marco Island, FL | 16,413 |
| Matlacha, FL | 677 |
| Matlacha Isles-Matlacha Shores, FL | 229 |
| McGregor, FL | 7,406 |
| Montura, FL | 3,283 |
| Moore Haven, FL | 1,680 |
| Naples, FL | 19,537 |
| Naples Manor, FL | 5,562 |
| Naples Park, FL | 5,967 |
| Nokomis, FL | 3,167 |
| North Fort Myers, FL | 39,407 |
| North Port, FL | 57,357 |
| Olga, FL | 1,952 |
| Orangetree, FL | 4,406 |
| Page Park, FL | 514 |
| Palmona Park, FL | 1,146 |
| Pelican Bay, FL | 6,346 |
| Pine Island Center, FL | 1,854 |
| Pine Manor, FL | 3,428 |
| Pine Ridge, FL | 1,918 |
| Pineland, FL | 407 |
| Pioneer, FL | 697 |
| Plantation, FL | 4,919 |
| Plantation Island, FL | 163 |
| Port Charlotte, FL | 54,392 |
| Port LaBelle, FL | 3,530 |
| Punta Gorda, FL | 16,641 |
| Punta Rassa, FL | 1,750 |
| Rotonda, FL | 8,759 |
| San Carlos Park, FL | 16,824 |
| Sanibel, FL | 6,469 |
| Sarasota County rural, FL | 80,864 |
| Solana, FL | 742 |
| South Venice, FL | 13,949 |
| Southeast Arcadia, FL | 6,554 |
| St. James City, FL | 3,784 |
| Suncoast Estates, FL | 4,384 |
| Three Oaks, FL | 3,592 |
| Tice, FL | 4,470 |
| Venice, FL | 20,748 |
| Venice Gardens, FL | 7,104 |
| Verona Walk, FL | 1,782 |
| Villas, FL | 11,569 |
| Vineyards, FL | 3,375 |
| Warm Mineral Springs, FL | 5,061 |
| Whiskey Creek, FL | 4,655 |
| | |
| Total | 1,481,945 |
