| City | Population |
| Fort Madison, IA | 11,051 |
| Keokuk, IA | 10,780 |
| Lee County rural, IA | 10,414 |
| Montrose, IA | 898 |
| Adams County rural, IL | 19,831 |
| Augusta village, IL | 587 |
| Bardolph village, IL | 251 |
| Barry, IL | 1,318 |
| Basco village, IL | 98 |
| Baylis village, IL | 205 |
| Beardstown, IL | 6,123 |
| Bentley town, IL | 35 |
| Bluffs village, IL | 715 |
| Bowen village, IL | 494 |
| Brown County rural, IL | 4,226 |
| Camden village, IL | 86 |
| Camp Point village, IL | 1,132 |
| Carthage, IL | 2,605 |
| Clayton village, IL | 709 |
| Coatsburg village, IL | 147 |
| Colchester, IL | 1,401 |
| Columbus village, IL | 99 |
| Detroit village, IL | 83 |
| El Dara village, IL | 78 |
| Elvaston village, IL | 165 |
| Exeter village, IL | 65 |
| Ferris village, IL | 156 |
| Florence village, IL | 38 |
| Georgetown, IL | 404 |
| Glasgow village, IL | 141 |
| Golden village, IL | 644 |
| Griggsville, IL | 1,226 |
| Hamilton, IL | 2,951 |
| Hancock County rural, IL | 6,248 |
| Hull village, IL | 461 |
| Industry village, IL | 478 |
| Kinderhook village, IL | 216 |
| La Harpe, IL | 1,235 |
| La Prairie village, IL | 47 |
| Liberty village, IL | 516 |
| Lima village, IL | 163 |
| Littleton village, IL | 181 |
| Loraine village, IL | 313 |
| Macomb, IL | 19,288 |
| McDonough County rural, IL | 5,861 |
| Mendon village, IL | 953 |
| Meredosia village, IL | 1,044 |
| Milton village, IL | 271 |
| Mound Station village, IL | 122 |
| Mount Sterling, IL | 2,025 |
| Nauvoo, IL | 1,149 |
| Nebo village, IL | 340 |
| New Canton town, IL | 359 |
| New Salem village, IL | 137 |
| Payson village, IL | 1,026 |
| Perry village, IL | 397 |
| Pike County rural, IL | 5,585 |
| Pittsfield, IL | 4,576 |
| Plainville village, IL | 264 |
| Plymouth village, IL | 505 |
| Quincy, IL | 40,633 |
| Ripley village, IL | 86 |
| Rushville, IL | 3,192 |
| Schuyler County rural, IL | 3,948 |
| Tennessee village, IL | 115 |
| Time village, IL | 23 |
| Ursa village, IL | 626 |
| Versailles village, IL | 478 |
| Warsaw, IL | 1,607 |
| West Point village, IL | 178 |
| Alexandria, MO | 159 |
| Ashburn town, MO | 52 |
| Baring, MO | 132 |
| Bethel village, MO | 122 |
| Bowling Green, MO | 5,334 |
| Canton, MO | 2,377 |
| Center, MO | 508 |
| Clarence, MO | 813 |
| Clark County rural, MO | 3,785 |
| Curryville, MO | 225 |
| Edina, MO | 1,176 |
| Ewing, MO | 456 |
| Frankford, MO | 323 |
| Hannibal, MO | 17,916 |
| Hunnewell, MO | 184 |
| Hurdland, MO | 163 |
| Kahoka, MO | 2,078 |
| Knox City, MO | 216 |
| Knox County rural, MO | 2,190 |
| La Belle, MO | 660 |
| La Grange, MO | 931 |
| Leonard village, MO | 61 |
| Lewis County rural, MO | 5,155 |
| Lewistown town, MO | 534 |
| Louisiana, MO | 3,364 |
| Luray village, MO | 99 |
| Marion County rural, MO | 7,270 |
| Monroe City, MO | 2,531 |
| Monticello village, MO | 98 |
| New London, MO | 974 |
| Newark village, MO | 94 |
| Novelty village, MO | 139 |
| Palmyra, MO | 3,595 |
| Perry, MO | 693 |
| Pike County rural, MO | 7,958 |
| Plevna, MO | 21 |
| Ralls County rural, MO | 7,764 |
| Rensselaer village, MO | 228 |
| Revere town, MO | 79 |
| Rutledge town, MO | 109 |
| Shelbina, MO | 1,704 |
| Shelby County rural, MO | 2,937 |
| Shelbyville, MO | 552 |
| South Gorin town, MO | 91 |
| St. Francisville, MO | 179 |
| Stoutsville village, MO | 36 |
| Tarrants village, MO | 22 |
| Wayland, MO | 533 |
| Wyaconda, MO | 227 |
| | |
| Total | 270,649 |
