| City | Population |
| Ackley, IA | 1,589 |
| Alburnett, IA | 673 |
| Allison, IA | 1,029 |
| Amana, IA | 442 |
| Anamosa, IA | 5,533 |
| Andrew, IA | 434 |
| Aplington, IA | 1,128 |
| Aredale, IA | 74 |
| Arlington, IA | 429 |
| Asbury, IA | 4,170 |
| Atkins, IA | 1,670 |
| Aurora, IA | 185 |
| Baldwin, IA | 109 |
| Balltown, IA | 68 |
| Bankston, IA | 25 |
| Belle Plaine, IA | 2,534 |
| Benton County rural, IA | 8,560 |
| Bernard, IA | 112 |
| Bertram, IA | 294 |
| Black Hawk County rural, IA | 9,761 |
| Blairstown, IA | 692 |
| Brandon, IA | 309 |
| Bremer County rural, IA | 6,924 |
| Brighton, IA | 652 |
| Bristow, IA | 160 |
| Brooklyn, IA | 1,468 |
| Buchanan County rural, IA | 7,788 |
| Butler County rural, IA | 5,588 |
| Cascade, IA | 2,159 |
| Castalia, IA | 173 |
| Cedar Falls, IA | 39,260 |
| Cedar Rapids, IA | 126,326 |
| Center Junction, IA | 111 |
| Center Point, IA | 2,421 |
| Central City, IA | 1,257 |
| Centralia, IA | 134 |
| Chelsea, IA | 267 |
| Chickasaw County rural, IA | 5,095 |
| Clarence, IA | 974 |
| Clarksville, IA | 1,439 |
| Clayton, IA | 43 |
| Clayton County rural, IA | 8,853 |
| Clermont, IA | 632 |
| Clutier, IA | 213 |
| Coggon, IA | 658 |
| Colesburg, IA | 404 |
| Conroy, IA | 259 |
| Coralville, IA | 18,907 |
| Deep River, IA | 279 |
| Delaware, IA | 159 |
| Delaware County rural, IA | 8,340 |
| Delhi, IA | 460 |
| Delta, IA | 328 |
| Denver, IA | 1,780 |
| Dike, IA | 1,209 |
| Dubuque, IA | 57,637 |
| Dubuque County rural, IA | 18,421 |
| Dumont, IA | 637 |
| Dundee, IA | 174 |
| Dunkerton, IA | 852 |
| Durango, IA | 22 |
| Dyersville, IA | 4,058 |
| Dysart, IA | 1,379 |
| Earlville, IA | 812 |
| East Amana, IA | 56 |
| Edgewood, IA | 864 |
| Elberon, IA | 196 |
| Elgin, IA | 683 |
| Elk Run Heights, IA | 1,117 |
| Elkader, IA | 1,273 |
| Elkport, IA | 37 |
| Ely, IA | 1,776 |
| Epworth, IA | 1,860 |
| Evansdale, IA | 4,751 |
| Fairbank, IA | 1,113 |
| Fairfax, IA | 2,123 |
| Farley, IA | 1,537 |
| Farmersburg, IA | 302 |
| Fayette, IA | 1,338 |
| Fayette County rural, IA | 6,989 |
| Franklin County rural, IA | 3,556 |
| Fredericksburg, IA | 931 |
| Frederika, IA | 183 |
| Frytown, IA | 165 |
| Garber, IA | 88 |
| Garnavillo, IA | 745 |
| Garrison, IA | 371 |
| Garwin, IA | 527 |
| Gibson, IA | 61 |
| Gilbertville, IA | 712 |
| Gladbrook, IA | 945 |
| Graf, IA | 79 |
| Greeley, IA | 256 |
| Greene, IA | 1,130 |
| Grundy Center, IA | 2,706 |
| Grundy County rural, IA | 4,268 |
| Guernsey, IA | 63 |
| Guttenberg, IA | 1,919 |
| Hampton, IA | 4,461 |
| Hansell, IA | 98 |
| Harper, IA | 114 |
| Hartwick, IA | 86 |
| Hawkeye, IA | 449 |
| Hayesville, IA | 50 |
| Hazleton, IA | 823 |
| Hedrick, IA | 764 |
| Hiawatha, IA | 7,024 |
| High Amana, IA | 115 |
| Hills, IA | 703 |
| Holiday Lake, IA | 433 |
| Holland, IA | 282 |
| Holy Cross, IA | 374 |
| Homestead, IA | 148 |
| Hopkinton, IA | 628 |
| Hudson, IA | 2,282 |
| Independence, IA | 5,966 |
| Iowa City, IA | 67,862 |
| Iowa County rural, IA | 6,295 |
| Jackson County rural, IA | 8,167 |
| Janesville, IA | 930 |
| Jesup, IA | 2,520 |
| Johnson County rural, IA | 21,273 |
| Jones County rural, IA | 8,730 |
| Kalona, IA | 2,363 |
| Keokuk County rural, IA | 3,883 |
| Keota, IA | 1,009 |
| Keswick, IA | 246 |
| Keystone, IA | 622 |
| Kinross, IA | 73 |
| La Motte, IA | 260 |
| La Porte City, IA | 2,285 |
| Ladora, IA | 283 |
| Lamont, IA | 461 |
| Lincoln, IA | 162 |
| Linn County rural, IA | 21,037 |
| Lisbon, IA | 2,152 |
| Lone Tree, IA | 1,300 |
| Luana, IA | 269 |
| Luxemburg, IA | 240 |
| Luzerne, IA | 96 |
| Manchester, IA | 5,179 |
| Marengo, IA | 2,528 |
| Marion, IA | 34,768 |
| Marquette, IA | 375 |
| Martelle, IA | 255 |
| Martinsburg, IA | 112 |
| Masonville, IA | 127 |
| Maynard, IA | 518 |
| McGregor, IA | 871 |
| Mechanicsville, IA | 1,146 |
| Middle Amana, IA | 581 |
| Millersburg, IA | 159 |
| Millville, IA | 30 |
| Monmouth, IA | 153 |
| Monona, IA | 1,549 |
| Monticello, IA | 3,796 |
| Montour, IA | 249 |
| Morley, IA | 115 |
| Morrison, IA | 94 |
| Mount Auburn, IA | 150 |
| Mount Vernon, IA | 4,506 |
| New Hampton, IA | 3,571 |
| New Hartford, IA | 516 |
| New Vienna, IA | 407 |
| Newhall, IA | 875 |
| North Buena Vista, IA | 121 |
| North English, IA | 1,041 |
| North Liberty, IA | 13,374 |
| Norway, IA | 545 |
| Oelwein, IA | 6,415 |
| Olin, IA | 698 |
| Ollie, IA | 215 |
| Onslow, IA | 197 |
| Ossian, IA | 845 |
| Osterdock, IA | 59 |
| Oxford, IA | 807 |
| Oxford Junction, IA | 496 |
| Packwood, IA | 204 |
| Palo, IA | 1,026 |
| Parkersburg, IA | 1,870 |
| Parnell, IA | 193 |
| Peosta, IA | 1,377 |
| Plainfield, IA | 436 |
| Postville, IA | 2,227 |
| Poweshiek County rural, IA | 5,470 |
| Prairieburg, IA | 178 |
| Quasqueton, IA | 554 |
| Randalia, IA | 68 |
| Raymond, IA | 788 |
| Readlyn, IA | 808 |
| Reinbeck, IA | 1,664 |
| Richland, IA | 584 |
| Rickardsville, IA | 182 |
| Riverside, IA | 993 |
| Robins, IA | 3,142 |
| Rowley, IA | 264 |
| Ryan, IA | 361 |
| Sageville, IA | 122 |
| Shell Rock, IA | 1,296 |
| Shellsburg, IA | 983 |
| Sherrill, IA | 177 |
| Shueyville, IA | 577 |
| Sigourney, IA | 2,059 |
| Solon, IA | 2,037 |
| South Amana, IA | 159 |
| South English, IA | 212 |
| Springville, IA | 1,074 |
| St. Donatus, IA | 135 |
| St. Lucas, IA | 143 |
| St. Olaf, IA | 108 |
| Stanley, IA | 125 |
| Stanwood, IA | 684 |
| Stone City, IA | 192 |
| Stout, IA | 224 |
| Strawberry Point, IA | 1,279 |
| Sumner, IA | 2,028 |
| Swisher, IA | 879 |
| Tama, IA | 2,877 |
| Tama County rural, IA | 6,858 |
| Thornburg, IA | 67 |
| Tiffin, IA | 1,947 |
| Toledo, IA | 2,341 |
| Traer, IA | 1,703 |
| Tripoli, IA | 1,313 |
| University Heights, IA | 1,051 |
| Urbana, IA | 1,458 |
| Van Horne, IA | 682 |
| Victor, IA | 893 |
| Vining, IA | 50 |
| Vinton, IA | 5,257 |
| Volga, IA | 208 |
| Wadena, IA | 262 |
| Walford, IA | 1,463 |
| Walker, IA | 791 |
| Washburn, IA | 876 |
| Washington, IA | 7,266 |
| Washington County rural, IA | 7,998 |
| Waterloo, IA | 68,406 |
| Watkins, IA | 118 |
| Waucoma, IA | 257 |
| Waverly, IA | 9,874 |
| Webster, IA | 88 |
| Wellman, IA | 1,408 |
| West Amana, IA | 135 |
| West Branch, IA | 2,322 |
| West Chester, IA | 146 |
| West Union, IA | 2,486 |
| Westgate, IA | 211 |
| What Cheer, IA | 646 |
| Williamsburg, IA | 3,068 |
| Winthrop, IA | 850 |
| Worthington, IA | 401 |
| Wyoming, IA | 515 |
| Zwingle, IA | 91 |
| East Dubuque, IL | 1,704 |
| Galena, IL | 3,429 |
| Menominee village, IL | 248 |
| Scales Mound village, IL | 376 |
| The Galena Territory, IL | 1,058 |
| Bagley village, WI | 379 |
| Bloomington village, WI | 735 |
| Cassville village, WI | 947 |
| Dickeyville village, WI | 1,061 |
| Fennimore, WI | 2,497 |
| Glen Haven, WI | 73 |
| Grant County rural, WI | 18,332 |
| Hazel Green village, WI | 1,256 |
| Kieler, WI | 497 |
| Lancaster, WI | 3,868 |
| Mount Hope village, WI | 225 |
| Patch Grove village, WI | 198 |
| Platteville, WI | 11,224 |
| Potosi village, WI | 688 |
| Prairie du Chien, WI | 5,911 |
| Sandy Hook, WI | 309 |
| Tennyson village, WI | 355 |
| | |
| Total | 906,648 |
