| City | Population |
| Alleghany County rural, NC | 9,385 |
| Eden, NC | 15,527 |
| Milton town, NC | 166 |
| Rockingham County rural, NC | 54,641 |
| Sparta town, NC | 1,770 |
| Wentworth town, NC | 2,807 |
| Alleghany County rural, VA | 10,843 |
| Allisonia, VA | 117 |
| Altavista town, VA | 3,450 |
| Amherst County rural, VA | 18,837 |
| Amherst town, VA | 2,231 |
| Appomattox County rural, VA | 13,021 |
| Appomattox town, VA | 1,733 |
| Bassett, VA | 1,100 |
| Bedford, VA | 6,222 |
| Bedford County rural, VA | 58,569 |
| Belspring, VA | 256 |
| Belview, VA | 891 |
| Big Island, VA | 303 |
| Blacksburg town, VA | 42,620 |
| Blairs, VA | 916 |
| Blue Ridge, VA | 3,084 |
| Boones Mill town, VA | 239 |
| Botetourt County rural, VA | 20,447 |
| Brookneal town, VA | 1,112 |
| Buchanan town, VA | 1,178 |
| Buena Vista, VA | 6,650 |
| Callaghan, VA | 348 |
| Campbell County rural, VA | 35,208 |
| Carroll County rural, VA | 23,527 |
| Cave Spring, VA | 24,922 |
| Charlotte County rural, VA | 10,455 |
| Charlotte Court House town, VA | 543 |
| Chatham town, VA | 1,269 |
| Chatmoss, VA | 1,698 |
| Christiansburg town, VA | 21,041 |
| Clifton Forge town, VA | 3,884 |
| Clover, VA | 438 |
| Cloverdale, VA | 3,119 |
| Cluster Springs, VA | 811 |
| Collinsville, VA | 7,335 |
| Concord, VA | 1,458 |
| Covington, VA | 5,961 |
| Craig County rural, VA | 5,037 |
| Daleville, VA | 2,557 |
| Danville, VA | 43,055 |
| Drakes Branch town, VA | 530 |
| Draper, VA | 320 |
| Dublin town, VA | 2,534 |
| East Lexington, VA | 1,463 |
| Elliston, VA | 902 |
| Fairlawn, VA | 2,367 |
| Fancy Gap, VA | 237 |
| Ferrum, VA | 2,043 |
| Fieldale, VA | 879 |
| Fincastle town, VA | 353 |
| Floyd County rural, VA | 14,854 |
| Floyd town, VA | 425 |
| Forest, VA | 9,106 |
| Fort Chiswell, VA | 939 |
| Franklin County rural, VA | 41,835 |
| Fries town, VA | 484 |
| Galax, VA | 7,042 |
| Giles County rural, VA | 10,454 |
| Glasgow town, VA | 1,133 |
| Glen Lyn town, VA | 115 |
| Glenvar, VA | 976 |
| Grayson County rural, VA | 13,924 |
| Gretna town, VA | 1,267 |
| Halifax County rural, VA | 23,029 |
| Halifax town, VA | 1,309 |
| Henry County rural, VA | 36,015 |
| Henry Fork, VA | 1,234 |
| Hillsville town, VA | 2,681 |
| Hiwassee, VA | 264 |
| Hollins, VA | 14,673 |
| Horse Pasture, VA | 2,227 |
| Hurt town, VA | 1,304 |
| Independence town, VA | 947 |
| Iron Gate town, VA | 388 |
| Ivanhoe, VA | 551 |
| Keysville town, VA | 832 |
| Lafayette, VA | 449 |
| Laurel Park, VA | 675 |
| Laymantown, VA | 1,979 |
| Lexington, VA | 7,042 |
| Low Moor, VA | 258 |
| Lynchburg, VA | 75,568 |
| Madison Heights, VA | 11,285 |
| Martinsville, VA | 13,821 |
| Max Meadows, VA | 562 |
| Merrimac, VA | 2,133 |
| Montgomery County rural, VA | 21,597 |
| Montvale, VA | 698 |
| Motley, VA | 1,015 |
| Mount Hermon, VA | 3,966 |
| Mountain Road, VA | 1,100 |
| Narrows town, VA | 2,029 |
| Nathalie, VA | 183 |
| New Castle town, VA | 153 |
| New River, VA | 244 |
| North Shore, VA | 3,094 |
| Oak Level, VA | 857 |
| Parrott, VA | 435 |
| Patrick County rural, VA | 15,237 |
| Pearisburg town, VA | 2,786 |
| Pembroke town, VA | 1,128 |
| Penhook, VA | 801 |
| Phenix town, VA | 226 |
| Pittsylvania County rural, VA | 53,769 |
| Plum Creek, VA | 1,524 |
| Prices Fork, VA | 1,066 |
| Pulaski County rural, VA | 19,100 |
| Pulaski town, VA | 9,086 |
| Radford, VA | 16,408 |
| Rich Creek town, VA | 774 |
| Ridgeway town, VA | 742 |
| Riner, VA | 859 |
| Riverdale, VA | 956 |
| Roanoke, VA | 97,032 |
| Roanoke County rural, VA | 43,707 |
| Rockbridge County rural, VA | 19,350 |
| Rocky Mount town, VA | 4,799 |
| Rural Retreat town, VA | 1,483 |
| Rustburg, VA | 1,431 |
| Salem, VA | 24,802 |
| Sandy Level, VA | 484 |
| Scottsburg town, VA | 119 |
| Selma, VA | 529 |
| Shawsville, VA | 1,310 |
| Snowville, VA | 149 |
| South Boston town, VA | 8,142 |
| Stanleytown, VA | 1,422 |
| Stuart town, VA | 1,408 |
| Timberlake, VA | 12,183 |
| Troutville town, VA | 431 |
| Union Hall, VA | 1,138 |
| Villa Heights, VA | 717 |
| Vinton town, VA | 8,098 |
| Virgilina town, VA | 154 |
| Westlake Corner, VA | 976 |
| Woodlawn, VA | 2,343 |
| Wythe County rural, VA | 17,489 |
| Wytheville town, VA | 8,211 |
| | |
| Total | 1,201,929 |
