| City | Population |
| Alachua, FL | 9,059 |
| Alachua County rural, FL | 99,113 |
| Archer, FL | 1,118 |
| Bell town, FL | 456 |
| Belleview, FL | 4,492 |
| Beverly Hills, FL | 8,445 |
| Black Diamond, FL | 1,101 |
| Bradford County rural, FL | 21,503 |
| Branford town, FL | 712 |
| Bronson town, FL | 1,113 |
| Brooker town, FL | 338 |
| Cedar Key, FL | 702 |
| Chiefland, FL | 2,245 |
| Citrus County rural, FL | 41,930 |
| Citrus Hills, FL | 7,470 |
| Citrus Springs, FL | 8,622 |
| Columbia County rural, FL | 50,824 |
| Cross City town, FL | 1,728 |
| Crystal River, FL | 3,108 |
| Dixie County rural, FL | 14,525 |
| Dunnellon, FL | 1,733 |
| East Bronson, FL | 1,945 |
| East Williston, FL | 694 |
| Five Points, FL | 1,265 |
| Fort White town, FL | 567 |
| Gainesville, FL | 124,354 |
| Gilchrist County rural, FL | 14,086 |
| Hampton, FL | 500 |
| Hawthorne, FL | 1,417 |
| Hernando, FL | 9,054 |
| High Springs, FL | 5,350 |
| Homosassa, FL | 2,578 |
| Homosassa Springs, FL | 13,791 |
| Horseshoe Beach town, FL | 169 |
| Inglis town, FL | 1,325 |
| Interlachen town, FL | 1,403 |
| Keystone Heights, FL | 1,350 |
| La Crosse town, FL | 360 |
| Lake Butler, FL | 1,897 |
| Lake City, FL | 12,046 |
| Lecanto, FL | 5,882 |
| Levy County rural, FL | 22,755 |
| Marion County rural, FL | 261,261 |
| McIntosh town, FL | 452 |
| Micanopy town, FL | 600 |
| Morriston, FL | 164 |
| Newberry, FL | 4,950 |
| Ocala, FL | 56,315 |
| Otter Creek town, FL | 134 |
| Pine Ridge, FL | 9,598 |
| Raleigh, FL | 373 |
| Reddick town, FL | 506 |
| Silver Springs Shores, FL | 6,539 |
| Spring Ridge, FL | 398 |
| Starke, FL | 5,449 |
| Steinhatchee, FL | 1,047 |
| Sugarmill Woods, FL | 8,287 |
| Trenton, FL | 1,999 |
| Union County rural, FL | 13,202 |
| Waldo, FL | 1,015 |
| Watertown, FL | 2,829 |
| Williston, FL | 2,768 |
| Williston Highlands, FL | 2,275 |
| Worthington Springs town, FL | 181 |
| Yankeetown town, FL | 502 |
| | |
| Total | 883,969 |
