| City | Population |
| Admire, KS | 156 |
| Allen, KS | 177 |
| Alma, KS | 832 |
| Alta Vista, KS | 444 |
| Americus, KS | 894 |
| Anderson County rural, KS | 3,487 |
| Auburn, KS | 1,227 |
| Belvue, KS | 205 |
| Burlingame, KS | 934 |
| Burlington, KS | 2,674 |
| Bushong, KS | 34 |
| Carbondale, KS | 1,437 |
| Chase County rural, KS | 1,272 |
| Circleville, KS | 170 |
| Coffey County rural, KS | 3,099 |
| Cottonwood Falls, KS | 903 |
| Council Grove, KS | 2,182 |
| Delia, KS | 169 |
| Denison, KS | 187 |
| Douglas County rural, KS | 11,907 |
| Dunlap, KS | 30 |
| Dwight, KS | 272 |
| Effingham, KS | 546 |
| Elmdale, KS | 55 |
| Emmett, KS | 191 |
| Emporia, KS | 24,916 |
| Eskridge, KS | 534 |
| Fort Riley, KS | 7,761 |
| Franklin County rural, KS | 9,107 |
| Geary County rural, KS | 8,919 |
| Grandview Plaza, KS | 1,560 |
| Grantville, KS | 180 |
| Harris, KS | 51 |
| Hartford, KS | 371 |
| Harveyville, KS | 236 |
| Havensville, KS | 133 |
| Herington, KS | 2,526 |
| Holton, KS | 3,329 |
| Hoyt, KS | 669 |
| Jackson County rural, KS | 8,131 |
| Jefferson County rural, KS | 12,186 |
| Junction City, KS | 23,353 |
| Lawrence, KS | 87,643 |
| Lebo, KS | 940 |
| Lecompton, KS | 625 |
| Leonardville, KS | 449 |
| Louisville, KS | 188 |
| Lyndon, KS | 1,052 |
| Lyon County rural, KS | 6,100 |
| Madison, KS | 701 |
| Manhattan, KS | 52,281 |
| Maple Hill, KS | 620 |
| Mayetta, KS | 341 |
| McFarland, KS | 256 |
| McLouth, KS | 880 |
| Melvern, KS | 385 |
| Meriden, KS | 813 |
| Milford, KS | 530 |
| Morris County rural, KS | 63 |
| Muscotah, KS | 176 |
| Neosho Rapids, KS | 265 |
| Netawaka, KS | 143 |
| New Strawn, KS | 394 |
| Nortonville, KS | 637 |
| Ogden, KS | 2,087 |
| Olivet, KS | 67 |
| Olpe, KS | 546 |
| Olsburg, KS | 219 |
| Onaga, KS | 702 |
| Osage City, KS | 2,943 |
| Osage County rural, KS | 6,791 |
| Oskaloosa, KS | 1,113 |
| Ottawa, KS | 12,649 |
| Overbrook, KS | 1,058 |
| Ozawkie, KS | 645 |
| Parkerville, KS | 59 |
| Paxico, KS | 221 |
| Perry, KS | 929 |
| Pomona, KS | 832 |
| Pottawatomie County rural, KS | 11,455 |
| Quenemo, KS | 388 |
| Reading, KS | 231 |
| Riley, KS | 939 |
| Riley County rural, KS | 7,435 |
| Rossville, KS | 1,151 |
| Scranton, KS | 710 |
| Shawnee County rural, KS | 46,292 |
| Silver Lake, KS | 1,439 |
| Soldier, KS | 136 |
| St. George, KS | 639 |
| St. Marys, KS | 2,627 |
| Strong City, KS | 485 |
| Topeka, KS | 127,473 |
| Valley Falls, KS | 1,192 |
| Vassar, KS | 530 |
| Wabaunsee County rural, KS | 3,910 |
| Wakarusa, KS | 260 |
| Wamego, KS | 4,372 |
| Waverly, KS | 592 |
| Welda, KS | 129 |
| Westmoreland, KS | 778 |
| Westphalia, KS | 163 |
| Wetmore, KS | 368 |
| Wheaton, KS | 95 |
| White City, KS | 618 |
| Whiting, KS | 187 |
| Willard, KS | 92 |
| Williamsburg, KS | 397 |
| Wilsey, KS | 153 |
| Winchester, KS | 551 |
| | |
| Total | 539,576 |
