| City | Population |
| Ashe County rural, NC | 24,213 |
| Avery County rural, NC | 15,204 |
| Bakersville town, NC | 464 |
| Banner Elk town, NC | 1,028 |
| Beech Mountain town, NC | 320 |
| Boone town, NC | 17,122 |
| Cove Creek, NC | 1,171 |
| Crossnore town, NC | 192 |
| Elk Park town, NC | 452 |
| Foscoe, NC | 1,370 |
| Grandfather village, NC | 25 |
| Jefferson town, NC | 1,611 |
| Lansing town, NC | 158 |
| Mitchell County rural, NC | 12,940 |
| Newland town, NC | 698 |
| Seven Devils town, NC | 192 |
| Sugar Mountain village, NC | 198 |
| Valle Crucis, NC | 412 |
| Watauga County rural, NC | 29,251 |
| West Jefferson town, NC | 1,299 |
| Baileyton town, TN | 431 |
| Banner Hill, TN | 1,497 |
| Bloomingdale, TN | 9,888 |
| Blountville, TN | 3,074 |
| Bluff City, TN | 1,733 |
| Bristol, TN | 26,702 |
| Carter County rural, TN | 34,909 |
| Central, TN | 2,279 |
| Church Hill, TN | 6,737 |
| Colonial Heights, TN | 6,934 |
| Elizabethton, TN | 14,176 |
| Erwin town, TN | 6,097 |
| Fall Branch, TN | 1,291 |
| Gray, TN | 1,222 |
| Greene County rural, TN | 48,313 |
| Greeneville town, TN | 15,062 |
| Hancock County rural, TN | 5,432 |
| Hawkins County rural, TN | 36,767 |
| Hunter, TN | 1,854 |
| Johnson City, TN | 63,152 |
| Johnson County rural, TN | 15,713 |
| Jonesborough town, TN | 5,051 |
| Kingsport, TN | 48,205 |
| Mosheim town, TN | 2,362 |
| Mount Carmel town, TN | 5,429 |
| Mountain City town, TN | 2,531 |
| Oak Grove, TN | 4,425 |
| Pine Crest, TN | 2,388 |
| Roan Mountain, TN | 1,360 |
| Rogersville town, TN | 4,420 |
| Sneedville town, TN | 1,387 |
| Spurgeon, TN | 3,957 |
| Sullivan County rural, TN | 53,936 |
| Surgoinsville town, TN | 1,801 |
| Telford, TN | 921 |
| Tusculum, TN | 2,663 |
| Unicoi County rural, TN | 7,087 |
| Unicoi town, TN | 3,632 |
| Walnut Hill, TN | 2,394 |
| Washington County rural, TN | 46,917 |
| Watauga, TN | 458 |
| Abingdon town, VA | 8,191 |
| Adwolf, VA | 1,530 |
| Appalachia town, VA | 1,754 |
| Atkins, VA | 1,143 |
| Big Stone Gap town, VA | 5,614 |
| Bristol, VA | 17,835 |
| Buchanan County rural, VA | 22,607 |
| Castlewood, VA | 2,045 |
| Cedar Bluff town, VA | 1,137 |
| Chilhowie town, VA | 1,781 |
| Claypool Hill, VA | 1,776 |
| Cleveland town, VA | 202 |
| Clinchco town, VA | 337 |
| Clinchport town, VA | 70 |
| Clintwood town, VA | 1,414 |
| Coeburn town, VA | 2,139 |
| Damascus town, VA | 814 |
| Dante, VA | 649 |
| Dickenson County rural, VA | 13,654 |
| Dryden, VA | 1,208 |
| Duffield town, VA | 91 |
| Dungannon town, VA | 332 |
| Emory, VA | 1,251 |
| Ewing, VA | 439 |
| Gate City town, VA | 2,034 |
| Glade Spring town, VA | 1,456 |
| Grundy town, VA | 1,021 |
| Haysi town, VA | 498 |
| Honaker town, VA | 1,449 |
| Jonesville town, VA | 1,034 |
| Keokee, VA | 416 |
| Lebanon town, VA | 3,424 |
| Lee County rural, VA | 19,782 |
| Marion town, VA | 5,968 |
| McMullin, VA | 464 |
| Meadow View, VA | 967 |
| Nickelsville town, VA | 383 |
| Norton, VA | 3,958 |
| Pennington Gap town, VA | 1,781 |
| Pound town, VA | 1,037 |
| Raven, VA | 2,270 |
| Richlands town, VA | 5,823 |
| Riverview, VA | 782 |
| Rose Hill, VA | 799 |
| Russell County rural, VA | 21,128 |
| Saltville town, VA | 2,077 |
| Scott County rural, VA | 18,940 |
| Seven Mile Ford, VA | 783 |
| Smyth County rural, VA | 17,704 |
| St. Charles town, VA | 128 |
| St. Paul town, VA | 970 |
| Sugar Grove, VA | 758 |
| Troutdale town, VA | 178 |
| Vansant, VA | 470 |
| Washington County rural, VA | 42,197 |
| Weber City town, VA | 1,327 |
| Wise County rural, VA | 25,870 |
| Wise town, VA | 3,286 |
| | |
| Total | 892,082 |
